A time-saving method for screening cDNA or genomic libraries
نویسندگان
چکیده
منابع مشابه
A time-saving method for screening cDNA or genomic libraries.
The common procedure for isolating genes of interest is to screen cDNA or genomic libraries with nucleic acid probes by filter hybridization (1). However, this is a time consuming and laborintensive process, especially when the complexity of the library is great and there is a large number of bactenophage plaques to screen. Here we describe the use of the polymerase chain reaction (PCR) (2) to ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.9.2830